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Blog Details for "The Molecular Biology Blog"
The Molecular Biology Blog![]() The Molecular Biology Blog Tech tips, technology updates, news and comment from the molecular biology field Articles
Networking with LinkedIn and LabRoots
2008-12-02 11:56:00 You may have seen some of the different websites around for networking. I?m not talking about Facebook or Myspace, which are for social networking. Facebook and Myspace are great for keeping up with friends and sharing more personal details about your non-work life. For scientific networking, you want to be able to connect with people on ... More About: Communication , Networking , Linkedin
DIY Electrocompetent E. coli
2008-11-25 15:34:00 If you buy competent E.coli regularly, you’ll know that they are pretty expensive. So the cost of screwing up a cloning or transformation experiment is pretty high in terms of money, as well as your time and sanity! But you don’t need this extra worry because despite what their high commercial cost would suggest, making good quality ...
Science as Progress, and More on the Philosophy of Science
2008-11-18 11:22:00 Following up on my recent post about The Nature of Scientific Observation, I left two-thirds of Chalmers? book What is This Thing Called Science untouched, including discussions on Bayes? theorem and the New Experimentalism. More About: Philosophy , Books , Progress
Why You Shouldn?t Worry about Getting Results
2008-11-17 11:43:00 Everyone is worried about getting results, aren’t they? Results are what you need for success in science - they are essential for bringing the funding in. But focusing on results per se is not a good way to work because, as a scientist, you can’t “get” results. You can’t “make” them happen. Essentially in every experiment you ... More About: Productivity , Worry
Around the Blogs
2008-11-14 11:15:00 This week’s highlights from Around the Blogs include shakes in the lab, confused science journalists and brain tissue from stem cells… 23andMe’s Advanced Global Similarity Tool Dienekes takes a critical look at 23andMe’s new “global similarity” tool, which allows you to visually place your own genetic data in the context of genome-wide SNP data from over 1,000 ... More About: Careers
Fast-track your Ampicillin Plasmid Transformations
2008-11-13 12:02:00 Most of us use pretty standard transformation protocols for E.coli. Yours probably goes something like this: - Thaw the competent cells on ice - Add DNA - Electroporate (or incubate then heat shock for chemically competent cells) - Add rich medium (LB or SOC) - Incubate at 37°C (or appropriate temperature) for 30-60 minutes - Spread onto antibiotic plates That 30-60 minute ... More About: Fast , Track
7 Ingredients for a Successful Job Application
2008-11-11 01:23:00 In my current position, I had the opportunity to hire a new R&D scientist to join the team. I was excited at being able to build my team and take my time to find the perfect fit for our company. My experience in the process of hiring gave me a new perspective on all the reasons ... More About: Careers , Ingredients , Application
A Grad Student, a Postdoc, and a Professor are Walking?
2008-11-10 11:10:00 A grad student, a post-doc, and a professor are walking through a city park and they find an antique oil lamp. They rub it and a Genie comes out in a puff of smoke. The Genie says, “I usually only grant three wishes, so I’ll give each of you just one.” “Me first! Me first!” says the ... More About: Student , Walking
Around The Blogs
2008-11-07 12:17:00 Our highlights from the blogosphere this week include cloning woolly mammoths, the logistics of tissue culture entertainment and Google’s efforts to save the world. Back from the dead. Sandra Porter at Discovering Biology in a Digital World covers the recent cloning of some long frozen mice by a Japanese group, which could take us one step ... More About: Blogs
5 Bio-Related Apps for your Iphone/Ipod Touch
2008-11-04 15:38:00 If you’re one of the proud owners of an Ipod touch or an Iphone then there some great apps available for academics that makes life a bit easier. Molecules is an application that allows you to view PDB structure files, yes it’s the 3 dimensional protein structure just like on software like rasmol, deepview etc. on ... More About: Touch , Related
Transferable Job Skills in Science
2008-06-09 12:07:00 Relating to my post last week on jobs in industry, concerning the slight disconnect between jobs in academia versus industry — the NatureJobs podcast has a special episode this past week from NatureJobs: From Academia to Industry [mp3]. In it, Pfizer’s Anthony Harrison talks about transferable skills that could help you move from a university-based ... More About: Science , Careers , Skills
Around the Blogs
2008-06-06 09:47:00 In this week’s around the blogs: How to talk to your professor, fragrant E.coli and chromatography-inspired poetry. Short and Schweeeeet. The Female Science Professor gives some hilarious advice to students on the etiquette of talking to their professors. Flash DNA sequencing. This post at Discovering Biology In a Digital World flags up some neat flash animations of ... More About: Blogs
How to reduce your lab?s environmental impact
2008-06-05 09:49:00 Bioscientists are generally nature-lovers at heart, but the average bio lab is incredibly wasteful. Here are some ways to reduce your lab's environmental impact. More About: Environmental , Reduce
Thoughts on Industry Jobs
2008-06-04 11:46:00 Last week, Sandra at Discovering Biology in a Digital World had some interesting thoughts on Life science PhD’s as industrial strength technicians. What I thought interesting was this bit: This wasn’t for a technician job, but a few years ago, I was in the position of hiring someone to help me on an education project. I ... More About: Industry , Jobs , Careers , Thoughts
This Week?s Best of the Kit
2008-06-03 17:12:00 Lazy DNA ligation, low carbon footprint bunsens and back-to-basics E.coli... they're among our picks of the kit for this week. More About: Week
Birth of the Cell Doctrine
2008-06-02 12:09:00 As a general rule of thumb, it is recommended to be familiar with the history of one’s scientific field, and not merely the contemporary trends of thought. That’s generally why I liked The Birth of the Cell so much when I read it. Dissatisfied by the standard accounts of the origin of the cell doctrine, Henry ... More About: Books
Around the Blogs
2008-05-31 07:11:00 While I generally am a lurker on other people’s blogs (I admit it), I have a long list of blogs that I subscribe to in my Google Reader feed. And since it’s Friday, I thought it time again to share some of the postings from around the blogs that caught my eye. On the Five Stages ... More About: Blogs
Avoding the Lure of The Internet
2008-05-31 07:10:00 The internet is a great tool, but can also be a real source of distraction. Here are five ways to avoid it's lure while working, and stay productive. More About: Internet , Productivity , The Internet , Lure
Around the Blogs
2008-05-30 12:57:00 While I generally am a lurker on other people’s blogs (I admit it), I have a long list of blogs that I subscribe to in my Google Reader feed. And since it’s Friday, I thought it time again to share some of the postings from around the blogs that caught my eye. On the Five Stages ... More About: Blogs
Avoiding the lure of the internet
2008-05-29 07:39:00 The internet can be a great source of distraction when you are trying to work. Here are some ways to control your surfing habits and save valuable time. More About: Internet , The Internet , Lure
How I Chose a ?Grad School? (UK edition!)
2008-05-27 13:33:00 In the US it's called Grad School , but in the UK we just call it "doing a PhD". Nick gives his story on how he chose his PhD position along with some advice for those currently looking for a PhD. More About: Careers , Edition
How I Chose a Grad School
2008-05-26 12:24:00 A reader recently asked for an explanation “about choosing grad schools, taking the GREs, visiting campuses, speaking to potential advisors, and how you guys decided on where to go.” For me, to be honest, I think that I was astoundingly naive in my decision-making for where to go for graduate school. For starters, I didn’t know ... More About: Careers , School
Around the Blogs
2008-05-23 14:24:00 This week is dominated by career-related posts discussing life in science. Check ‘em out! Postdoc Malaise It’s hard to stay motivated when there feels like so many obstacles standing in the way of your career. Why It’s So Hard to Get That Course You Need A faculty member explains one of the frequent difficulties cited by students trying ... More About: Blogs
Scientist, GSOH. Seeks Similar.
2008-05-20 02:41:00 How does the lonely scientist find that special someone with whom they can share their copy of Molecular Biology of The Cell? More About: Scientist
Building Science in a Small Country
2008-05-19 13:51:00 Dan makes some interesting observations on the grant funding system in Cyprus, the country he recently relocated to. More About: Science , Building , Country , Careers , Small
Animated DNA replication
2008-05-19 07:24:00 A stunning animation of DNA replication More About: Animated
Around The Blogs
2008-05-16 13:01:00 Time for our weekly look around the best of the blogs… More on Google Reader. This article on the Bootstrapper blog, along with Bala’s article on Google Reader for Academics, will help you on your way to Google Reader mastery. All our ideas in one basket. Mr Gunn at Synthesis commented on a very interesting project, that ... More About: Blogs
How to Refold 653 Insoluble Proteins
More articles from this author:2008-05-15 07:22:00 Bacteria are good hosts for expressing recombinant proteins, mainly because they are easy to manipulate and grow. But their relatively simple expression systems can’t cope with every gene you throw at them so proteins will often fail to express properly. Sometimes the protein is fully expressed but cannot fold properly. This is particularly common when the ... 1, 2, 3, 4, 5, 6, 7 |




